Preparation and characterization of Aflatoxin M1- Fluoresceinamine conjugate for AFM1 immunoassay in milk
نویسندگان
چکیده
AFM1-oxime-Fluoresceinamine conjugate was prepared and chracterized. .For that purpose fist was obtained and purified AFM1-oxime. UV-Vis scan spectral analysis of the obtained AFM1oxime-Fluoresceinamine conjugate was made. The activity of AFM1-oxime-Fluorescenamine conjugate was measured. It was proved that the obtained conjugate had high activity and potential possibility for detection of AFM1 in milk by immunofluorescent assay.
منابع مشابه
Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin M1 in milk.
A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for detecting aflatoxin M1 (AFM1) was developed. To improve the sensitivity of the assay, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used to enhance peroxidase-induced CL. The concentrations of the coating anti-AFM1 antibody and the conjugate of AFB1 with h...
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